Overexpression of miR-34a in canine OSA8 cells significantly alters gene expression.

<p>Total RNA was isolated from canine OSA8 cells expressing control or pre-miR-34a lentiviral constructs from three separate transduction experiments and next-generation sequencing was performed to identify differences in gene transcript expression. Supervised hierarchical cluster analysis of 111 genes differentially expressed in OSA8 cells expressing either empty vector (EV) or miR-34a (miR34a) as determined by one-way ANOVA comparison test (<i>p</i> ≤ 0.0001). Color areas indicate relative expression of each gene after log2 transformation with respect to the gene median expression (red and green colors denote high and low expression, respectively).