Effect of ADAMTS5 genotype on AT during cold exposure for 72 h.

<p>(A, B) Gene expression levels of <i>Ucp-1</i> (A) and <i>Cidea</i> (B) in sWAT of WT (+/+) and homozygous deficient (-/-) mice from the ADAMTS5-P (+/+: n = 10; -/-: n = 8) and ADAMTS5-J strains (+/+: n = 11; -/-: n = 7). Gene expression levels are normalized to the housekeeping gene <i>Tbp</i> and shown relative to WT mice at +4°C. (C,D) UCP-1 protein levels in sWAT. The Western blots (C) are quantitated by densitometry, normalized to β-actin and expressed relative to WT (D). (E-G) Relative ADAMTS5 gene expression in sWAT (E), GON AT (F) and BAT (G) of WT mice kept at 24°C or at 4°C. Gene expression levels are normalized to the housekeeping gene <i>Tbp</i> and shown relative to WT mice at 24°C. Data are means ± SEM of 4–8 and 5–11 determinations for panel D and panel E-G, respectively. ** p<0.01, *** p<0.001 versus 24°C according to the non-parametric Mann-Whitney U test. ADAMTS5, a disintegrin and metalloproteinase with thrombospondin type 1 motifs member 5; WAT, white adipose tissue; UCP-1, uncoupling protein-1; s, subcutaneous; WT, wild-type; GON, gonadal; BAT, brown adipose tissue and <i>Tbp</i>, TATA-box binding protein.