miR6019/6020 family is conserved in the Solanaceae plant family.

<p>(A) Relative <i>N</i> transcript levels in tomato D51 (blue) and VF36 (red) plants at 1-, 3- and 6 WAG stages as determined by qRT-PCR. (B) Mature miR6019 and miR6020 sequences from different <i>Solanaceae</i> plants are paired to the TIR coding sequences of the <i>N</i> gene. The sequence logo of the TIR amino acid sequences encoded by the miR6019/6020 target sequence is shown. <i>N</i> sequences highlighted in red represent the binding sites for the miR6019 and miR6020 seed sequences. Polymorphic nucleotides in mature miR6020 are highlighted in red. (C) Secondary structures of the <i>N</i>. <i>benthamiana</i> and <i>S</i>. <i>lycopersicum</i> miR6019/6020 precursors. Mature miRNA and miR star are highlighted in red. (D) Levels of tomato miR6020 and secondary siRNAs derived from the coding region of <i>N</i> at 1-, 3- and 6 WAG stages. (E) Levels of tobacco secondary siRNAs derived from the coding region of <i>N</i> at 1-, 3- and 6 WAG stages. The data are the means of three replicates with SD. Different letters indicate significant differences between the treatments according to one-way ANOVA test (P < 0.05). The Y axes in A indicate relative fold. The Y axes in D and E are in TPM units.