REV-ERBα and REV-ERBβ ChIP-Seq signal is higher at CLOCK:BMAL1 DNA binding sites targeting genes transcribed at night.

<p>A-C <i>(Left)</i>. Average ChIP-Seq signal for REV-ERBα (A), REV-ERBβ (B) and E4BP4 (C) at CLOCK:BMAL1 DNA binding sites (center ± 1kb) for each of the 4 CLOCK:BMAL1 transcriptional output group. A-C (<i>Right)</i>. Distribution of REV-ERBα (A), REV-ERBβ (B) and E4BP4 (C) ChIP-Seq signal at CLOCK:BMAL1 peaks for each of the 4 CLOCK:BMAL1 transcriptional output groups (signal for each peak was averaged at CLOCK:BMAL1 peak center ± 250bp). Groups are labeled as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007156#pgen.1007156.g001" target="_blank">Fig 1</a>. Those with different letters are significantly different (Kruskal-Wallis test; p < 0.05). REV-ERBα and REV-ERBβ ChIP were performed from mice liver collected at ZT10, while E4BP4 ChIP was performed from mice liver collected at ZT22. ChIP-Seq datasets were retrieved from Cho et al., 2012 [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007156#pgen.1007156.ref035" target="_blank">35</a>], and E4BP4 ChIP-Seq datasets from Fang et al., 2014 [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007156#pgen.1007156.ref036" target="_blank">36</a>].