<p><b>Percent abundance of IgH D- (A) and J- (B) gene segments, IgH constant regions (C) and Igκ J-gene segments (D).</b> Sequencing reads corresponding to each gene segment or constant region were divided by the total number of gene segments or constant regions identified in each mouse pool for normalized comparison between pools (left side). The most abundant gene segment is ranked as one (right side). Dark red indicates higher rank moving to blue (A) or white (B-D), of lower rank. Sequencing reads designated undetermined (undeter) where portions of a D- or J-gene segment were identified but unable to be assigned to a specific C57BL/6J D- or J-gene segment.</p
Abstract Background To date several studies have sought to catalog the full suite of antibodies that...
<p>Analysis of IgE, IgG1, and IgM sequences from mediastinal LN of 3 mice 15 d after primary <i>N</i...
Summary: The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humo...
<p><b>V-gene segment usage among unimmunized mouse pools for IgH (A) and Igκ (B) by chromosomal loca...
<p><b>Combinations of V-gene families with DJ-gene segments for IgH (A) and J-gene segments for Igκ ...
Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segm...
Background Next-generation sequencing (NGS) of antibody variable regions has emerged as a powerful ...
<p>Genes that were in the top 10 contributors to the χ<sup>2</sup> value and constitute >2.5% of the...
Given the diversity and complexity within immunoglobulin (IG) loci, effective mouse models first req...
Immunoglobulin loci-transgenic animals are widely used in antibody discovery and increasingly in vac...
International audienceThe relationship between the immune repertoire and the physiopathological stat...
The V(D)J recombination process selects and rearranges the variable (V), diversity (D), and joining ...
<div><p>Variability in the developing antibody repertoire is focused on the third complementarity de...
Doctor of PhilosophyDivision of BiologyStephen ChapesAntibodies are an essential part of the immune ...
Automated gene-prediction procedures have a number of shortcomings, and a method has been developed ...
Abstract Background To date several studies have sought to catalog the full suite of antibodies that...
<p>Analysis of IgE, IgG1, and IgM sequences from mediastinal LN of 3 mice 15 d after primary <i>N</i...
Summary: The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humo...
<p><b>V-gene segment usage among unimmunized mouse pools for IgH (A) and Igκ (B) by chromosomal loca...
<p><b>Combinations of V-gene families with DJ-gene segments for IgH (A) and J-gene segments for Igκ ...
Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segm...
Background Next-generation sequencing (NGS) of antibody variable regions has emerged as a powerful ...
<p>Genes that were in the top 10 contributors to the χ<sup>2</sup> value and constitute >2.5% of the...
Given the diversity and complexity within immunoglobulin (IG) loci, effective mouse models first req...
Immunoglobulin loci-transgenic animals are widely used in antibody discovery and increasingly in vac...
International audienceThe relationship between the immune repertoire and the physiopathological stat...
The V(D)J recombination process selects and rearranges the variable (V), diversity (D), and joining ...
<div><p>Variability in the developing antibody repertoire is focused on the third complementarity de...
Doctor of PhilosophyDivision of BiologyStephen ChapesAntibodies are an essential part of the immune ...
Automated gene-prediction procedures have a number of shortcomings, and a method has been developed ...
Abstract Background To date several studies have sought to catalog the full suite of antibodies that...
<p>Analysis of IgE, IgG1, and IgM sequences from mediastinal LN of 3 mice 15 d after primary <i>N</i...
Summary: The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humo...