RNA-Seq analysis of the <i>det2-9</i> and WT root transcriptome.

<p>(A) Differential transcription in <i>det2-9</i> and WT. Genes, which were significantly induced or repressed in <i>det2-9</i> compared with WT, appear in, respectively, the upper left (in red) and bottom right (in green) hand portions of the plot. RPKM: reads per kilobase per million reads, DEGs: differentially expressed genes. (B, C) A heat map representation of the transcriptional behavior of genes associated with ethylene (B) and responding to ROS (C) based on the GO analysis. *: means more than two times up change in the expression genes of <i>det2-9</i> compared to WT. (D) A qRT-PCR expression analysis of a selection of genes related to ethylene in <i>det2-9</i> compared to WT. Data shown are mean±SE (n = 3), **: means of <i>det2-9</i> and WT differ significantly (<i>P</i><0.01).