Quantitative immunofluorescence for assessment of cellular composition.

<p>Two weeks post-injury VML affected and I-R injured muscles were separately probed for Pax7 and CD68 to assess locality and density of satellite cell and macrophage populations. A) Composite images of the muscle belly cross-sections were capture and digitally enumerated. B) Whole muscle and C) regional analysis of specified cellular infiltration were performed. *Indicates significant difference (p<0.05) relative to No Repair group. ^†Indicates significant main effect of group (p<0.05) by 2-way ANOVA of the specified independent variable. Values are means ± sem. Scale bars are 1 mm.