Expression of Wnt genes with anterior ectodermal localizations.

<p>Whole mount in situ hybridization of Wnt genes with ectodermal localization in the more anterior domain of the embryo. All data are presented as optical sagittal or frontal sections following clearing in Murray Clear. Developmental staging is from blastula to 72 h of development. All embryos are oriented with anterior, or animal (in the case of blastula), to the top left of the panel and posterior, or vegetal, to the bottom right of the panel. Right column, ventral is to the bottom left. Unless otherwise noted, expression is ectodermal. (Ai-Av), Expression of <i>wnt8</i> at blastula stage (Ai), midgastrula stage (Aii), 30 h (Aiii), 50 h, side view (Aiv), and 72 h of development, side view (Av). (Bi-Bv), Expression of <i>wnt 2</i>. Blastula stage (Bi), at late gastrula (Bii), at 36 h (Biii), at 48 h, frontal view (Biv), and at 72 h of development, side view (Bv). (Ci-Cv), Expression of <i>wnt11</i> at blastula stage (Ci), at gastrula stage (Cii), at 48 h, side view (Ciii), 60 h, side view (Civ), and 72 h of development, side view. (Di-Dv), Expression of <i>wnt7</i> at blastula stage (Di), gastrula stage (Dii), 36 h (Diii), 48 h, showing a dorsal view with focal plane through the dorsal ectoderm (Div), and 72 h of development, side view (Dv). (Ei-Ev), Expression of <i>wnt5</i> at blastula stage (Ei), at midgastrula stage (Eii), at 48 h, dorsal view (Eiii), at 60 h, side view (Eiv), and at 72 h of development in side view (Ev). (Fi-Fv), Expression of <i>wntA</i>, no expression for the first 48 h at blastula stage (Fi), gastrula stage (Fii), and at 40 h of development (Fiii). Expression begins at 48 h shown as a frontal section from a dorsal view (Fiv), and at 72 h of development with a side view (Fv). (Gi-Gv), Expression of <i>wnt9</i> at blastula stage (Gi), at midgastrula stage (Gii), at 48 h, showing a ventral view with optical section through the ventral ectoderm and posterior mesoderm (Giii), at 60 h of development again in ventral view (Giv), and at the same stage in side view (Gv).