Cancer sepsis impacts CD4⁺ T cell proliferation but not glucose uptake.

<p>CD4⁺ T cells isolated from splenocytes of previously healthy or cancer septic animals were A) incubated for 30 min ex vivo with fluorescently labeled 2-NBDG as described in Materials and Methods or B) stained intracellularly for Ki-67 as described in Materials and Methods. A, Representative flow plots of 2-NBDG uptake in CD4⁺ T cells cells isolated from previously healthy (blue histograms) vs. cancer septic (red histograms) mice. B, Summary data of frequencies of 2-NBDG⁺ CD4⁺ T cells. (C) Representative flow plots and D) summary data of Ki-67 staining in CD4⁺ T cells populations isolated from previously healthy vs. cancer septic mice. Summary data tabulated from n = 5/group. **p<0.01.