Expanded NK cells demonstrate increased cytotoxicity against HCT116 and HT29 cells as compared to fresh, IL-2 activated cells.

<p>The indicated NK cells were assessed for cytotoxic activity against target cancer cells using a calcein AM flow cytometry assay following 4 hours co-incubation. (A) Percentage cell death of HCT116 cells induced by NK cells at the indicated Effector:Target cell ratios. (B and C) Percentage HT29 cell death induced by NK cells from 3 individual healthy donors at Effector:Target ratios of 1:1 (B) and 4:1 (C). Bars represent data using IL2-activated NK cells (FRESH) and following expansion of NK cells from the same donor (EXPANDED). *p<0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001; ns p≥0.05.