Histology signature analysis across intrinsic subtypes of breast cancer.

<p>(A)Heatmap depicting expression of mouse mammary tumor derived histology signatures in the METABRIC dataset featuring each intrinsic subtype of breast cancer. Genes are illustrated my median centered, log2 normalized expression level. Samples were clustered within each intrinsic and then genes were clustered within each signature across tumors. (B) Analysis of the EMT signature (highly expressed in EMT) genes in basal-like human breast cancers shows that high expression of this signature is significantly associated with earlier incidence of tumor relapse. pval = 0.0108, HR = 2.05, N = 142. (C) Analysis of the adenomyoepithelial signature genes in basal-like human breast cancers shows that high expression of this signature is significantly associated with earlier incidence of tumor relapse. pval = 7e−04, HR = 2.63, N = 142. (D) Analysis of the adenomyoepithelial signature genes in luminal B human breast cancers shows that high expression of this signature is significantly associated with earlier incidence of tumor relapse. pval = 7e−04, HR = 2.63, N = 447. (E) Analysis of the solid signature genes (highly expressed in solid tumors) in luminal B human breast cancers shows that high expression of this signature is significantly associated with delayed incidence of tumor relapse. pval = 0.0159, HR = 0.721, N = 447. Kaplan-Meier analysis was done using the <a href="http://geneanalytics.duhs.duke.edu/Surv_sig.html" target="_blank">http://geneanalytics.duhs.duke.edu/Surv_sig.html</a> tool. Samples were assigned to groups based on being above or below the median population value.