Typical histological appearance in specimens at 2 weeks following transplantation onto the cranium.

<p>Coronal plane sections were stained with hematoxylin and eosin (H&E). For each group, the left panel shows the whole area of specimens (scale bars represent 1 mm), and the right panel shows the magnified image of the white box in the left panel (scale bars represent 200 μm). A small amount of new bone was detected along the host bone in the <b>(A)</b> PB, (<b>B</b>) PPP, <b>(C)</b> PRP, and <b>(E)</b> BM-PPP groups. The (<b>D</b>) BM and (<b>F</b>) BMC groups showed obvious bone formation along the host bone. <b>(G)</b> New bone area surrounded the β-TCP particles and connected with the host bone [box area in (F)]. <b>(H)</b> Masson’s trichrome staining showed the immature (blue) and mature (red) bone in the new bone area [box area in (F)]. (<b>I</b>) Human vimentin immuno-staining showed a few positive cells at the surface of newly formed bone adjacent to the β-TCP particles. The yellow dotted line indicates the boundary between the host bone and the specimen.