Identification of <i>rHSA</i> gene expression in transgenic silkworm pedigrees using qRT-PCR, SDS-PAGE and Western blot, and purification of rHSA protein from the cocoon shells.

<p><b>(A)</b> The relative expression levels of the <i>rHSA</i> gene in the PSGs of the transgenic silkworm pedigrees on the 3^rd day of the 5^th instar were measured by qRT-PCR. WT: the wild-type silkworm <i>Lan</i> 10. HSA-1, HSA-2, HSA-3, HSA-4 and HSA-5: the transgene-positive silkworm pedigrees. (<b>B</b>) SDS-PAGE analysis of rHSA derived from the soluble protein of cocoon shells and (<b>C</b>) Western blot analysis of the cocoon layer. WT: the protein samples from the wild-type silkworm <i>Lan</i> 10. HSA-1, HSA-2, HSA-3, HSA-4 and HSA-5: the protein samples from the transgene-positive silkworm pedigrees. (<b>D</b>) The results of purification of the rHSA protein. S: the protein sample. W1, W2: recovered solutions after adding binding buffer. E1-E4: recovered solutions after adding elution buffer.