Effects of WPCD on BM-DCs function through TLR4-mediated pathway.

<p>After pretreatment with 5 μM of TAK-242 for 1 h, BM-DCs were treated with 100 ng/mL LPS or 100 and 200 μg/mL WPCD for 12 h in the presence or absence of Golgi Stop. Then CD40, CD86, TNF-a and IL-12 expressions were detected by FACs or ELISA. The MFI values of CD40 <b>(A)</b> and CD86 <b>(B)</b>, the yields of TNF-a <b>(C)</b>, and IL-12 <b>(D)</b> were shown. Data represent mean ± SD (n = 3) from 3 independent experiments.*<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001 compared with the untreated DCs group.