Inhibition of eIF2α phosphatase rescues mGluR-LTD, but not synaptically-induced LTD.

<p>The summary graphs in the right panels show mean ± SEM for the final 5 min of recording. <b>(A)</b> LTD after treatment with group 1 agonist DHPG (100 μM, applied during the gap in the graph) was reduced in <i>Thap1</i>^<i>+/-</i> slices, and pretreatment with Sal003 (20 μM) (eIF2α phosphatase inhibitor) rescued LTD in <i>Thap1</i>^<i>+/-</i> slices. <b>(B)</b> In high frequency stimulation (HFS)-induced LTD, Sal003 (10 μM) failed to reverse the deficit observed in <i>Thap1</i>^<i>+/-</i> slices. Numbers in parentheses indicate number of slices/number of mice. Representative traces are shown during baseline period (solid lines) and at the end of the recording period (dashed lines). Calibrations: 1 mV / 5 ms. Asterisks indicate <i>p</i> < .05 (*) or p < .01 (**) by ANOVAs followed by Newman-Keuls <i>post-hoc</i> tests. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007169#pgen.1007169.s012" target="_blank">S9 Table</a>.