Activity of autologous plasma against cell-cell transmission of CAP256 viruses is strongly driven by VRC26 bnAb activity.

<p><b>A:</b> 50% neutralizing titers (in plasma dilution) of autologous CAP256 plasma from week 145 p.i. against free virus (black) and cell-cell transmission (red) of CAP256 virus strains is depicted. Data are means of 2 to 3 independent experiments. Inhibition of <i>SU-like VRC26 sensitive viruses</i> and <i>SU-like VRC26 early escape</i> viruses in free virus and cell-cell transmission were compared using the Mann-Whitney test. P values are indicated in black and red respectively. <b>B:</b> The change in neutralization activity for cell-cell compared to free virus transmission is shown as the ratio of NT50^free-virus/NT50^cell-cell (Fold change Neutralizing titer). Zero denotes equal activity in both transmission pathways. A 10-fold higher activity in free virus over cell-cell transmission reflects a log₁₀ = 1, a 10-fold lower activity a log₁₀ = -1 as indicated by dotted lines. Viruses that were not neutralized to 50% at the half lowest plasma concentration were denoted as resistant (res.) and their fold changes were estimated. <b>C:</b> Interrelations of neutralizing titers for plasma and IC50s for bnAb neutralization were determined separately for PI-like and SU-like viruses during free virus and cell-cell transmission. <b>D:</b> Interrelations of virus infectivity in free virus and cell-cell transmission, IC50s and neutralizing titers (NT50) were determined for SU-like viruses. <b>C+D:</b> Spearman correlations on untransformed data sets were used, R and p values are indicated. Orange fields negative correlations. N.s. denotes no significance.