Bovine lactoferrin induced expression of SOCS3 to attenuate JAK2/STAT3 activation involving in inhibition of growth OSCC cells.

<p>HSC3 cells were treated with bLF (1 μg/ml, 10 μg/ml, and 100 μg/ml) for 24 h for mRNA and 48 h for protein expressions. (<b>A</b>) Expression of SOCS3 was found to be enhanced after bLF treatment checked by RT-PCR and western blot. (<b>B</b>) Protein levels of p-JAK2 and p-STAT3 were examined by western blot. bLF inhibited the activation of JAK/STAT3 pathway in a dose-dependent manner. (<b>C</b>) Number of HSC3 cells were counted under presence or absence of S3I-201 (50 μM) and bLF (100 μg/ml). Either S3I-201 or bLF-S3I-201 remarkably reduced number of HSC3 cells. On the other hand, no significant suppressive effect of bLF on HSC3 S3I-201 treated cells was observed. (<b>D</b>) G1/S cell cycle related molecules were analyzed by western blot in S3I-201 and S3I-201-bLF stimulated HSC3 cells. There were no significant differences in expression of p21 and cyclin D1 in HSC3 cells with S3I-201 and its combination groups. GAPDH and β-actin were used as a loading control. Images are representative of 3 independent experiments (n = 3).