Gene structure of <i>Sg-5</i> (<i>Glyma</i>.<i>15g243300</i>) and the polymorphisms between ′Pungsannamul′ and ′CWS5095′.

<p>(A) Mutational changes in the recessive alleles at the <i>Sg-5</i> locus are indicated in the mutant by the white boxes. Exons are indicated by gray boxes. The asterisk indicates the most influential SNP out of the four SNPs detected in ′CWS5095′. (B) Phylogenetic tree constructed using neighbor-joining clustering of 18 CYP450 superfamily proteins belong to different plant species. CYP450 superfamily proteins were classified into three groups (clades I to III). The Sg-5 protein was positioned in clade III and was distantly related to the clade I CYP450 superfamily. (C) Comparison of amino acid sequence Sg-5 proteins with 18 CYP450 superfamily proteins from plants (GenBank accession numbers are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192150#pone.0192150.s004" target="_blank">S4 Fig</a>). An important amino acid substitution detected in ′CWS5095′ is highlighted in black and its position is marked with an asterisk. The EXXR motif is denoted by a red box and identical amino acids are shown in gray.