Effect of mature adipocytes, their secretions and Tamoxifen (Tx) on cell proliferation.

<p>(A) MCF-7 cells were co-cultured with MA obtained from women of normal weight (MA20), overweight (MA27) or obese (MA30) women and treated with Tx. (B) 184B5 cells were co-cultured with MA27 with or without Tx. For A) and B), the proliferation was quantified after 72h using a resazurine test (Fluoroskan Ascent^® FL). (C) MCF-7 and (D) 184B5 cells were also cultured with conditioned media from the culture of MA20 (CM20) and AM30 (CM30) with or without Tx treatment. Results were expressed as Mean ± SEM, ¤ MA <i>vs</i> Control, * Tx <i>vs</i> Control, § MA+Tx <i>vs</i> Tx; $ CM30 <i>vs</i> CM20, £ CM+Tx <i>vs</i> CM, # CM30+Tx <i>vs</i> CM20+Tx (n = 3, Student's t test).