Intraperitoneal administration of MHV68 reveals a reactivation defect in the absence of ORF75A.

<p>C57BL/6 mice were infected at 1000 PFU by the intraperitoneal route with the indicated viruses. <b>(A)</b> Splenomegaly at 18 dpi with indicated viruses. Each symbol represents an individual mouse. Line indicates geometric mean titer. <b>(B)</b> Frequency of splenocytes harboring genomes 18 dpi. <b>(C)</b> Frequency of splenocytes spontaneously reactivating from latency 18 dpi. <b>(D)</b> Reactivation efficiency of splenocytes 18 dpi. <b>(E)</b> Frequency of PECs harboring genomes 18 dpi. <b>(F)</b> Frequency of PECs spontaneously reactivating from latency 18 dpi. For the limiting dilution analyses, curve fit lines were determined by nonlinear regression analysis. Using Poisson analysis, the intersection of the nonlinear regression curves with the dashed line at 63.2% was used to determine the frequency of cells that were either positive for the viral genome or reactivating virus. Data is generated from 5 independent experiments with 4–5 mice per group for splenocyte data and 2 independent experiments with 4 mice per group for PEC data. ** p ≤ 0.005, *** p ≤ 0.0005, and **** p ≤ 0.00005; for A significance determined by two-way unpaired t-test; for B-F significance determined by two-way paired t-test.