Loss of <i>Rbfox3</i> does not compromise the number of ganglion cells and amacrine cells in the retina.

<p>(a) Whole mount retinas from WT and KO mice immunostained with RBFOX3 antibody (green) and counter-stained with DAPI (blue). Scale bar = 1000 μm. (<b>b</b>) Schematic of four regions of mouse retina. Squares inside each region represent fields used for cell counts. Representative images and quantification in all four regions of retina in WT and KO mice: ganglion cells stained with anti-BRN3A antibody (<b>c</b>) and number of BRN3A-positive ganglion cells quantified (WT, n = 5 mice; KO, n = 5 mice) (<b>d</b>); amacrine cells stained with anti-Calbindin antibody (<b>e</b>) and number of Calbindin-positive amacrine cells quantified (Two-way ANOVA with Holm-Sidak <i>post hoc</i> comparison, *<i>P</i> < 0.05, **<i>P</i> < 0.01. WT, n = 4 mice; KO, n = 5 mice) (<b>f</b>); and anti-CHAT antibody with a focus on the GCL (upper panels) and INL (lower panels) (<b>g</b>) and quantification of ChAT-positive amacrine cells (WT, n = 3 mice; KO, n = 3 mice for both GCL and INL) (<b>h</b>). Scale bar = 20 μm. All data are presented as mean ± s.e.m. All data points were available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192355#pone.0192355.s009" target="_blank">S1 Table</a>.