<i>Rbfox3</i> deletion decreased the thickness of the IPL in the retina.

<p>(<b>a</b>) Left: H&E staining of the whole eyeball of WT and KO mice. Scale bar = 500 μm. Squares in (<b>a</b>) are areas of the enlarged images for panel (<b>b</b>). Scale bar = 50 μm. (<b>c</b>) The thickness of each layer of different regions of the retina was quantified by normalizing to the total thickness of the retina. n = 3 mice for WT and KO group. Two-way ANOVA with Holm-Sidak <i>post hoc</i> comparison, *<i>P</i> < 0.05, **<i>P</i> < 0.01. All data are presented as the mean ± s.e.m. OS = outer segment; IS = inner segment; ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer; IPL = inner plexiform layer; GCL = ganglion cell layer. Middle = the area around the midpoint between the optic nerve head (ONH) and the edge of retina closest to cornea. Central = the area around the midpoint between ONH and “Middle”. Peripheral = the area around the midpoint between the “Middle” and the edge of retina closest to cornea. All data points were available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192355#pone.0192355.s009" target="_blank">S1 Table</a>.