Biolistic coapplication of MOG- and cytokine-encoding plasmids ameliorates clinical outcomes of MOGp35-55-induced EAE.

<p>C57BL/6 mice were biolistically transfected with the indicated plasmid combinations in equimolar amounts 3 times in weekly intervals before induction of EAE. (A-C) Clinical symptoms were evaluated by EAE score for the following 16 days. For better clarity, data are shown in 3 graphs. Data of the reference group copGFP+CMV-GFP are the same in the 3 graphs. (D) Start of EAE symptoms, maximal EAE score and EAE incidence are depicted. N = 12 to 57 mice per group. *p<0.05, **p<0.01, ***p<0.001 versus copGFP+CMV-GFP mice, ⁺⁺p<0.01, ⁺⁺⁺p<0.001 versus non-vaccinated mice.