Intestinal RICT-1 regulates adult exploratory behaviors.

<p><b>A)</b> Cartoon depicting foraging assay. Each plate contains a L4 hermaphrodite which is allowed to forage overnight on a uniform lawn of <i>E</i>. <i>coli</i> for ~ 16hrs. Lines show tracks on the bacterial lawn. <b>B,E,F)</b> Exploratory behavior of indicated strains. Each purple dot represents data from a single worm. Median is indicated by a horizontal black line; error bars are quartiles. Light gray thin and thick vertical bars at right indicate Bayesian 95% and 75% credible intervals, respectively. Numbers in parentheses below indicate the total number of animals examined during 3 independent days of assays. <i>P</i>-values shown are between indicated values; ** and ***—different from WT at <i>P<</i>, respectively; ^^^—different from <i>rict-1</i> at <i>P</i><0.001 (Poisson GLMM with Tukey-type multivariate-t post-hoc adjustment). <i>P</i>-values of differences in means relative to wild-type and corresponding mutant animals are indicated in black and red, respectively. All transgenic experiments show data from a single transgenic line. <b>C)</b> Live tracking of foraging behavior. Shown is a representative assay. n > 20 animals per assay. Density plot shows mean speed and angular velocity from tracks binned over 10 sec intervals imaged at 3 frames/sec. Red line indicates delineation of roaming (upper region) and dwelling (lower region) behavior (slope = 2). <b>D)</b> Quantification of roaming and dwelling states. Dots show proportion of track time bins in which animals were roaming, with each dot reflecting one assay. n = 5 assays per genotype. Light gray thin and thick vertical bars at right indicate Bayesian 95% and 75% credible intervals, respectively. <i>P</i>-value shown is with respect to wild-type (Welch’s t-test).