Potensi Biosida Ekstrak Akar Dan Batang Pisang Kepok Untuk Pertumbuhan Biji Kacang Hijau Secara In Vitro

Microbial contamination is one of the major challenges hampering the application of in vitro micropropagationtechnique, to overcome the contamination used Plant Preservative Mixtured(PPM) as an antimicrobial bioside. Banana root and pseudo stem known have bioactive compounds such as flavonoid, saponin, dantanin can be used as antibacterials. This study aims to determine the ratio of potentialbiocide potential of exstract banana kepok root and pseudo stem for the growth of green beans the in vitro. Methodology used experimental method with completely randomized design with two factors: factor 1: Banana Root (P1), Banana Pseudo stem (P2) and factor 2: extract concentration of 0,5% (K1), extract concentration of 0,25% (K2). Exstraction banana kepok root and pseudo stem do with maseration method using ethanol solvent 70% temperature reaches maximum 90o C to get thickenedextract using destilator.Parameters observed for 7 days from green bean seeds were percentage of uncontaminated media, stem height, root number, leave number and germination condition. Condition of sprouts on P1K1, P1K2, P2K2, control negative as well control positive is normal. Extract of banana pseudo stem concentration 0,25% effectively prevent contamination by 60% on growth of green beans the in vitro