Proteomic profiling for potential biomarkers of osteosarcoma patients / Zulaika Roslan

Osteosarcoma (OS) is a common malignant bone tumour mainly occurs in children and young adults between the ages of 10 to 30 years. Although the advance of technology and improvement in chemotherapy treatment for OS has significantly improved the survival rate in recent years, its poor prognosis and unknown etiology continues to remain the major challenge in managing the disease progression. Therefore, the objectives of this study are to establish the serum protein profile of OS patients, to investigate the differential protein expression in pre and postchemotherapy and to identify the potential protein biomarkers relevant in OS cell migration. Serum and bone tissues biopsies were collected from healthy volunteers, pre- and post-chemotherapy OS patients. In this study, OS serum protein profile was established using a 4-plex iTRAQ analysis. Approximately 217 proteins with 104,214 spectra were acquired which includes low abundance proteins involved in biological processes and other protein classes. In addition, VCAM-1 was significantly altered (p