Small volume of hypertonic saline as the initial fluid replacement in experimental hypodynamic sepsis

Abstract\ud \ud \ud \ud Introduction\ud \ud We conducted the present study to examine the effects of hypertonic saline solution (7.5%) on cardiovascular function and splanchnic perfusion in experimental sepsis.\ud \ud \ud \ud Methods\ud \ud Anesthetized and mechanically ventilated mongrel dogs received an intravenous infusion of live Escherichia coli over 30 minutes. After 30 minutes, they were randomized to receive lactated Ringer's solution 32 ml/kg (LR; n = 7) over 30 minutes or 7.5% hypertonic saline solution 4 ml/kg (HS; n = 8) over 5 minutes. They were observed without additional interventions for 120 minutes. Cardiac output (CO), mean arterial pressure (MAP), portal and renal blood flow (PBF and RBF, respectively), gastric partial pressure of CO2 (pCO2; gas tonometry), blood gases and lactate levels were assessed.\ud \ud \ud \ud Results\ud \ud E. coli infusion promoted significant reductions in CO, MAP, PBF and RBF (approximately 45%, 12%, 45% and 25%, respectively) accompanied by an increase in lactate levels and systemic and mesenteric oxygen extraction (sO2ER and mO2ER). Widening of venous-arterial (approximately 15 mmHg), portal-arterial (approximately 18 mmHg) and gastric mucosal-arterial (approximately 55 mmHg) pCO2 gradients were also observed. LR and HS infusion transiently improved systemic and regional blood flow. However, HS infusion was associated with a significant and sustained reduction of systemic (18 ± 2.6 versus 38 ± 5.9%) and mesenteric oxygen extraction (18.5 ± 1.9 versus 36.5 ± 5.4%), without worsening other perfusional markers.\ud \ud \ud \ud Conclusion\ud \ud A large volume of LR or a small volume of HS promoted similar transient hemodynamic benefits in this sepsis model. However, a single bolus of HS did promote sustained reduction of systemic and mesenteric oxygen extraction, suggesting that hypertonic saline solution could be used as a salutary intervention during fluid resuscitation in septic patients.The authors thank the Department of Bacteriology of the Adolfo Lutz Institute, São Paulo, for providing a strain of live E. coli to develop the experimental model. This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil. FAPESP – grant 98/15658-0.The authors thank the Department of Bacteriology of the Adolfo Lutz Institute, São Paulo, for providing a strain of live E. coli to develop the experimental model. This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil. FAPESP – grant 98/156580