In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine
- TAPAJOS, E. C. C.
- LONGO, J. P.
- SIMIONI, A. R.
- LACAVA, Z. G. M.
- SANTOS, M. F. M. A.
- MORAIS, P. C.
- TEDESCO, A. C.
- AZEVEDO, R. B.
DOIAbstract
In this study, oral carcinoma cells were used to evaluate chloroaluminum-phthalocyanine encapsulated in liposomes as the photosensitizer agent in support of photodynamic therapy (PDT). The genotoxicity and cytotoxicity behavior of the encapsulated photosensitizer in both dark and under irradiation using the 670-nm laser were investigated with the classical trypan blue cell viability test, the acridine orange/ethidium bromide staining organelles test, micronucleus formation frequency, DNA fragmentation, and cell morphology. The cell morphology investigation was carried out using light and electronic microscopes. Our findings after PDT include reduction in cell viability (95%) associated with morphologic alterations. The neoplastic cell destr...
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