This dissertation explores a line of philosophical thought that accords negation a fundamental role in the determination of concepts and the kinds, universals and particulars to which concepts provide access. Though it takes the largely historical route of philosophical genealogy, focusing on Plato, Boethius and Hegel, the dissertation is also intended as a defense of the philosophical significance of negation itself, and of a principle Hegel famously attributes to Spinoza, omnis determinatio est negatio. I argue that in explaining negation as difference (to heteron) Plato reveals its originary function as the characteristic operation of cognition, and that this discursive role is the basis of the logical and grammatical roles it is more co...