HISTOLOGI TESTIS MENCIT (Mus musculus L.) MUDA DAN TUA YANG DIBERI EKSTRAK LADA HITAM (Piper nigrum L)

Black pepper (Piper nigrum L.) is traditionally believed to have some benerfits. In addition to keep healthy for users. Black pepper is also known to have some antioxidant properties by capturing free radical contribute to aging process. The experiment was conducted using mice with the aim to determine the effect of black pepper on histological changes of testis and the number of spermatozoa between old and young mice group. The experiments used 20 male mice age of 4 months and 20 mice aged of 6 months. Each of the age group were divided into 4 treatment groups. Group 1 as control group, without any black pepper extract. Group 2, were given water extract of black pepper with concentration of 0.3 ppt. Group 3 were given ethanol extract of black pepper with concentration 0.3 ppt, and Group 4 were given mixture of black pepper extract either with water and ethanol with concentration of 0.3 ppt. Data were analyzed with Two-way Analysis of Variance (ANOVA) (with α=5%). The results showed that the extract of black pepper treated on old mice increase the amount of spermatogonia with an average of 8.16 spermatogonial celss compared with the untreated group (with cell number of 47.0) in one incision of seminiferous tubules. While the number of spermatozoa were not significantly different from among groups.\ud \ud Key words: mice (Mus musculus L), black pepper (Piper nigrum L), spermatogonia, spermatozoa\u