Isolation and characterization of microsatellite loci in the deep-sea marine fish, the roundnose grenadier (Coryphaenoides rupestris)

We developed polymerase chain reaction primers for eight dinucleotide microsatellite lociin the marine deep sea fish, roundnose grenadier (Coryphaenoides rupestris). All markerswere obtained from a partial genomic DNA library, and characterized in 90 unrelated individualsfrom one putative population sampled on the Mid-Atlantic Ridge. The number ofalleles ranged from two to 61 with an average of 21 per locus. The observed heterozygositylevels ranged from 0.301 to 0.987 with an average of 0.672. Several of the markers amplifiedmultiple alleles from either the Atlantic cod (Gadus morhua) or the deep-sea fish roughheadgrenadier (Macrourus berglax)