Simultaneous determination of vanillylmandelic and homovanillic acids in urine using LC with coulometric electrochemical detection based on SPE sample preparation

The aim of the study was to develop a simple and selective LC method for the simultaneous measurement of vanillylmandelic acid (VMA) and homovanillic acid(HVA) in human urine. A method for the measurement of VMA and HVA using LC with coulometric electrochemical detection and investigation into the extraction techniques with regard to stability and recovery are described. The analytical performance of presented method is satisfactory. The presented method uses a relatively rapid SPE procedure. It can be used both for research and for clinical testing purposes