This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray assays. The assays utilize the optical characteristics of colloidal gold nanoparticles for the colorimetric detection of proteins. Multiplexed detection in sandwich immunoassays is limited by cross-reactivity between different detection antibodies. The cross-reactivity between antibodies can contribute to increased background noise - decreasing the Limit-of-Detection of the assay - or generate false positive signals. Paper I shows improved assay sensitivity in a multiplexed vertical flow assay by the application of ultrasonic energy to the gold nanoparticles functionalized with detection antibodies. The ultrasonication of the antibody conjug...
Development of state-of-the-art assays for sensitive and specific detection of disease biomarkers ha...
Gold nanoparticles (AuNPs) have unique optical and chemical properties. Dynamic light scattering (DL...
This dissertation outlines the design, fabrication, and optimization of microfluidic systems for the...
This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray...
Lateral flow assays are popular diagnostic tools which are constantly increasing in demand. However,...
As early diagnosis is essential for improving patient outcomes when treating both communicable and n...
Most analytical techniques that are routinely used in biomedical research for detection and quantifi...
As early diagnosis is essential for improving patient outcomes when treating both communicable and n...
Most analytical techniques that are routinely used in biomedical research for detection and quantifi...
In this work, we report a highly sensitive colorimetric sensing strategy for cancer biomarker diagno...
Thesis (Ph.D.)--University of Washington, 2018The simple and inexpensive sandwich-immunoassay-based ...
Gold nanoparticles have gained interest as theranostic platforms because their surface can be easily...
Thesis (Ph.D.)--University of Washington, 2018The simple and inexpensive sandwich-immunoassay-based ...
The enzyme-linked immunosorbent assay (ELISA) technique is based on the specific recognition ability...
Metal nanoparticles are particles made up of many individual metal atoms ranging in size from one na...
Development of state-of-the-art assays for sensitive and specific detection of disease biomarkers ha...
Gold nanoparticles (AuNPs) have unique optical and chemical properties. Dynamic light scattering (DL...
This dissertation outlines the design, fabrication, and optimization of microfluidic systems for the...
This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray...
Lateral flow assays are popular diagnostic tools which are constantly increasing in demand. However,...
As early diagnosis is essential for improving patient outcomes when treating both communicable and n...
Most analytical techniques that are routinely used in biomedical research for detection and quantifi...
As early diagnosis is essential for improving patient outcomes when treating both communicable and n...
Most analytical techniques that are routinely used in biomedical research for detection and quantifi...
In this work, we report a highly sensitive colorimetric sensing strategy for cancer biomarker diagno...
Thesis (Ph.D.)--University of Washington, 2018The simple and inexpensive sandwich-immunoassay-based ...
Gold nanoparticles have gained interest as theranostic platforms because their surface can be easily...
Thesis (Ph.D.)--University of Washington, 2018The simple and inexpensive sandwich-immunoassay-based ...
The enzyme-linked immunosorbent assay (ELISA) technique is based on the specific recognition ability...
Metal nanoparticles are particles made up of many individual metal atoms ranging in size from one na...
Development of state-of-the-art assays for sensitive and specific detection of disease biomarkers ha...
Gold nanoparticles (AuNPs) have unique optical and chemical properties. Dynamic light scattering (DL...
This dissertation outlines the design, fabrication, and optimization of microfluidic systems for the...