Hyperthermia and cisplatin increase heat shock protein heme oxygenase-1 expression in ovcar-3 cells

Aims To evaluate the impact of different temperature and cisplatin combinations to the expression of heat shock protein heme-oxygenase-1 (HO-1) and the cells viability in OVCAR-3 cells. Method Imitating the typical clinical conditions of HIPEC, OVCAR-3 cells were exposed to different hyperthermia and cisplatin concentrations for one hour. Afterward the MTT viability test, flow cytometer analysis, isobologram analysis and quantitative reverse transcription polymerase chain reaction was performed and analysed. Results The rising temperature from 37°C to 42°C alone and in combination with half of the maximal inhibitory concentration of cisplatin (IC50) had insignificant effect on OVCAR-3 cells viability and apoptosis. The combination of hyperthermia up to 42°C and IC50 dose of cisplatin significantly increased the expression of heat shock protein HO-1 in OVCAR-3 cells. Cisplatin alone decreased cell viability in a linear pattern. The antagonistic effect of hyperthermia and cisplatin was revealed by the isobologram method. Conclusion Hyperthermia usable in clinical practise and cisplatin increase the expression of heat shock protein HO-1 in OVCAR-3 cells. Further research is essential regarding modulation of the HO-1 expression as a new therapeutic option to improve the results of HIPEC