Matrix Metalloproteinase-8 Gene Polymorphism In Oral Cancer Development

Introduction. Oral cancer is the eighth most commonly diagnosed cancer worldwide generally affected older men’s [1]. MMP-8 (collagenase-2) is the most effective collagenase to initiate type I collagen degradation [2]. High MMP-8 expression level has been reported to be protective in human tongue cancer and in a carcinogeninduced mice model [3]. One study of the rs11225395 single-nucleotide polymorphism (SNP) at the MMP8 promoter region may affect the expression levels of MMP-8 has been done in the Taiwanese population, however results were not statistically significant [4]. According to those findings, we aimed to determine the association between MMP-8 rs11225395 and oral cancer development in the Lithuanian population. Materials and methods: Our study involved 859 participants including 27 patients with oral cancer and 832 healthy persons from control group. Peripheral blood samples from all participants were collected into EDTA collection tubes. DNA was extracted using DNA salting-out method. Genotyping was carried out using realtime polymerase chain reaction (RT-PCR) method. Statistical analysis was performed with „SPSS version 20.0“. Results. Statistical analysis showed that MMP-8 rs11225395 genotypes (GG, GA, AA) distribution analysis revealed statistically significant differences between oral cancer patients and control groups (22,2%, 51,9%, 25,9 vs. 30,5%, 48,9%, 20,6%, respectively, p<0.001). Binomial logistic regression analysis showed that genotype A/A at rs11225395 compared with is associated with 1,3-fold increased risk of oral cancer development under the recessive genetic model ((OR=1,347; 95%. CI: 0,560-3,238; p=0,043). Binomial logistic regression analysis also showed that male gender older age associated with increased risk of oral cancer development (OR=5,471; 95%. CI: 2,185-13,699; p