Quantitative determination of polymerase chain reaction product for HIV-1

A method to detect and quantify polymerase chain reaction (PCR) product from a DNA target fragment associated with Acquired Immunodeficiency Syndrome (AIDS) was developed. The current method to detect PCR product is by Agarose Gel Electrophoresis and staining with ethidium bromide, but quantitation is by the operator\u27s estimation. As a result of this project, the PCR product can be detected by High Pressure Liquid Chromatography (HPLC) and the amount of product can be determined by using an internal standard. By detection of the PCR product on the HPLC and subsequent quantitation, the efficiency of the PCR can be determined. Samples from different PCR experiments (all using the HIV-1 target fragment) were tested using the HPLC method to determine the product\u27s concentration