EVALUATION OF ANTI-UROLITHIATIC POTENTIAL OF NOVEL SIDDHA FORMULATION SEENAKARAPARPAM ON ETHYLENE GLYCOL-INDUCED UROLITHIASIS IN WISTAR ALBINO RATS

Urinary calculi are the third prevalent disorder of the urinary system. Approximately 80% of these calculi are composed of calcium oxalate and calcium phosphate. In India, urolithiasis constitutes one of the commonest afflictions requiring surgical intervention and by conservative estimates there are about 5–7 million patients suffering from urinary calculus disease in India. It is not only the higher prevalent disease condition which requires early attention, but rather the more problematic disorder with, higher rate of recurrence after surgical removal. The main aim of the present study is to investigate the anti-urolithiatic activity of Seenakaraparpam (SKP) on ethylene glycol-induced urolithiasis in wistar albino rats. All the experimental animals except normal control received ethylene glycol (0.75%) in drinking water for a period of 28 days for induction of urolithiasis. Rat belongs to treatment group co-administered with SKP at the dose of 100,200 and 300 mg/kg b.wt, p.o from 1st to 28st day, Animals belongs to standard group received cystone 500 mg/kg, p.o. Parameters such as volume and urinary biochemical parameters were measured after 14 & 28 days, Blood was collected on the 28st day for biochemical estimation. Treatment with SKP significantly reduced the excretion of oxalate and Ca and phosphate, protein, uric acid and creatinine in compared to lithiatic control group.The results of the study also shows that serum parameters such as calcium, uric acid, creatinine, oxalate, phosphate levels were increased significantly in animals belongs to lithiatic control. Treatment with SKP at the dose of 100, 200 and 300mg/kg and standard drug cystone at the dose of 500 mg/kg shown significant decrease in serum calcium, uric acid, creatinine, oxalate, phosphate levels. The level of magnesium was restored near to normal in treatment group animals. In conclusion formulations like SKP may be considered as a potential lead for the clinical management of urolithiasi