The Antimicrobial Efficiency of Aqueous Solutions of Calcium Hydroxide on Streptococcus mutans, Streptococcus faecalis and Candida albicans, in vitro

Preparati kalcijevog hidroksida mogu biti pripravljeni u obliku vodene otopine, kita, laka, cementa i umjetnog materijala. Terapijski učinak pojedinog preparata ovisi o fizikalno-kemijskim svojstvima dodanih sastojaka. Cilj rada bio je ispitati osjetljivost mikroorganizama Streptococcus mutans, Streptococcus faecalis i Candida albicans na različite koncentracije vodenih suspenzija kalcijevog hidroksida. Za kruta hranilišta korišteni su Bacto Mitis Salivarius agar za S. mutans, »Torlak« krvni agar za S. faecalis i Sabouraud agar »Torlak« za C. albicans. Na hranilišta je stavljeno 0,2 ml pripremljene suspenzije mikroorganizama. Na podlogama su izbušene udubine u koje je stavljeno po 0,2 .ml vodene otopine kalcijevog hidroksida. Tako pripremljena hranilišta držana su u aerobnim uvjetima na 3TC kroz 48 sati. Rezultati su očitavani nakon 24 i 48 sati. Ispitivane otopine kalcijevog hidroksida pokazale su maksimalni antimikrobni učinak nakon 24 sata. Rezultat je ostao isti i nakon 48 sati. Na krutom hranilištu inhibicija rasta S. mutans nastupila je kod koncentracije otopine Ca(OH) 2 od 271,18 x IO\u27 3 mol/dm i pH 12,76. Inhibicija rasta S. faecalis nastala je kod koncentracije od 3335,21 x 10 mol/dm3 i pH 12,82. Inhibicija rasta C. albicans nastupila je kod koncentracije otopine Ca(OH) 2 od 1023,20 x IO\u27 3 mol/dm3 i pH 12,84. Na pokusima in vitro zamijećena je razlika u osjetljivosti ispitivanih mikroorganizama kod istih koncentracija otopina Ca(OH)2.Calcium hydroxide preparations Ca(OH) 2 are used as water solutions, putly, liner, cement or arteficial material. The therapeutic effect of each medicaments depends on physical and chemical haracteristic of additional components. The aim of the paper is to research the sensitivity of Streptococcus faecalis and Candida albicans to different water solution concentrations o f calcium hydroxide. As solid plates, Bacto-Mitis-Salivarius agar was used for S. mutans, Torlak blood agar for S. faecalis and Sabourand agar »Torlak« for C. albicans. The holes were drilled in solid plates and were filled by 0 . 2 ml o f water solutions of Ca(OH)2. Preparations, created as described were incubated aerobically at 3TC in the period o f 48 hrs. Results were read after 24 as well as after 48 hrs. The examined solutions of Ca(OH)2, revealed the highest antimicrobic effect after 24 hrs. The result remained unchanged even after 48 hrs. The inhibition of S. mutans growth on the solid plate occured with the concentration o f Ca(OH) 2 solution from 271,18 x 10\u272 mol/dm3 and pH 12,76. The growth of S. faecalis at the solid plate was inhibited with the concentration of 3335,21 x 10\u27 3 mol/dm3 and pH 12,82. The inhibition of C. albicans growth on solid plate occured with the concentration o f Ca(OH) 2 solution from 1023,20 x 10\u273 molldm3 and pH 12,84. The difference in the sensitivity o f the examined microorganisms at the same concentrations of Ca(OH) 2 solution was observed