ESTIMATION OF GENETIC DIVERSITY USING PROBABILITIES OF GENE ORIGIN OF AUTOCHTHONOUS CATTLE BREED BUSHA

Registar izvorne pasmine goveda buša utemeljen je 2003. i danas broji 1020 grla. U cjelokupnom rodovniku utvrđeno je 329 foundera i 249 polufoundera. Broj foundera u referentnoj populaciji iznosi 285, polufoundera 219 i 255 ancestora, a 50% genetske varijabilnosti objašnjava 17 ancestora i 20 foundera. Najveći broj poznatih generacija predaka u rodovniku je pet, s prosječnim ekvivalentom kompletnih generacija 1,41 koji ukazuje na slabu informativnost i kompletnost rodovnika. Dio značajnih predaka je dao svoj doprinos genetskoj varijabilnosti kao founder, ali ne i kao ancestor, što ukazuje na gubitak dijela genetske varijabilnosti. Povećanje stupnja inbreedinga i povezanosti, omjeri pojedinih parametara porijekla gena (fe/fa, fg/fe, fe/f) i smanjenje doprinosa broja foundera u referentnoj populaciju u odnosu na cjelokupan rodovnik, ukazuju na gubitak genetske varijabilnosti u populaciji. Rezultati analize rodovnika ukazuju da je u cilju očuvanja genetske varijabilnosti, nužno i žurno dizajniranje kvalitetnog uzgojnog programa, plana pripusta i konzervacijske strategije, temeljenih na analizama rodovnika i metodama molekularne genetike (DNA).Register of autochthonous cattle breed busha was founded in 2003. and till today it is consisted of 1020 heads. In whole pedigree 329 founders and 249 half-founders are determined. The number of founders in reference population is 285, half-founders 219, and ancestors 255, and 50% of genetic variability explain 17 ancestors and 20 founders. The largest number of complete generations 1,41 which indicates poor informativity and completness of pedigree. Part of significant ancestors gave its contribution to genetic variability as founder, but not as ancestor which indicates loss of part of genetic variability. Increasing of inbreeding rate and connections, ratios of certain parameters of gene origin (fe/fa, fg/fe, fe/f) and decreasing of contribution of number of founders in reference population compared to the whole pedigree, indicate the loss of genetic variability in population. Pedigree analysis results indicate that in purpose of preservation of genetic variability, it is necessary and urgent creating of quality breeding progam, breeding plan and conservation strategy, based on pedigree analysis and method of molecular genetic