Does PKC-beta II Modulate Colon Cell Growth?

It has been shown that Protein Kinase C Beta II (PKCβII) is over expressed and more active in colon carcinoma tissues in comparison to surrounding non-involved tissues. A study is being conducted to determine if PKCβII is a causative step in or effect of colon tumor formation. The PKCβII deoxyribose-nucleic acid (DNA) sequence has been sub-cloned into an Entry vector with a inducible promoter. Site-directed mutagenesis had been carried out on the PKCβII kinase domain in order to allow for studies to be conducted on the role of PKCβII kinase activity in colon tumors. The PKCβII has been sub-cloned from the Entry vector into a pT-Rex-DEST31 vector also with a inducible promoter. KPNI restriction enzyme has been used to verify that PKCβII is present in the correct locations of the pT-Rex-DEST31 plasmid