Razina plazmatičnog kortizola u nazimica tretiranih imunostimulatorom virusnog podrijetla (Parapoxvirus ovis) u kasnoj fazi gravidnosti.

The goal of these investigations was to check if two-time treatment of gilts by Baypamun® before transferring from the pre-farrowing to farrowing unit can activate natural killer cells, stimulate leukocyte production and lymphocyte proliferation, and decrease the rise in plasma cortisol caused by stress. The investigation comprised 30 one year-old Swedish Landrace gilts in the late phase of gravidity, which were divided into three groups. Group 1 formed a control group, from blood which was taken in the the pre-farrowing unit in order to enable comparison of the results of plasma cortisol with results obtained in the other two groups. In group 2, Baypamun in a dose of 2 ml i/m at days 7 and 5 was applied, and in group 3 at days 3 and 1 prior to transferral from the pre-farrowing to farrowing unit. After gilts in groups 2 and 3 had been transferred four days prior to farrowing, blood was taken from them at days 1, 3 and 5 of their stay in the farrowing unit. Plasma cortisol was analyzed by the radioimmunochemical (RIA) method. The obtained results by groups were compared with each other using one-way analysis of variance. According to the obtained results an average level of plasma cortisol of the gilts in groups 2 and 3 throughout the research was higher than in control group 1. A significant statistical difference (P < 0.05) appeared between control group 1 and group 3 at day 1 of the stay of gilts in the farrowing unit. Therefore, an anti-stress effect of Baypamun occurred between days 1 and 3 of the stay in the farrowing unit, i.e. by the day of farrowing, when the level of plasma cortisol again began to rise due to stress caused by partus.Cilj ovih istraživanja bio je provjeriti može li dvokratno tretiranje nazimica Baypamunom prije premještanja iz čekališta u prasilište aktivirati prirodne stanice ubojice, potaknuti stvaranje leukocita i proliferaciju limfocita te sniziti porast kortizola uzrokovan stresom. Istraživanje je provedeno na 30 jednogodišnjih nazimica pasmine švedski landras u kasnoj fazi gravidnosti podijeljenih u tri skupine. Prva skupina bila je kontrolna. Drugoj skupini nazimica dano je 2 ml Baypamuna im. 7. i 5. dana prije preseljenja iz čekališta u prasilište, a trećoj skupini 3. i 1. dana prije preseljenja. Nakon što su nazimice iz druge i treće skupine preseljene, njima je prvog, trećeg i petog dana boravka u prasilištu izvađena krv. Plazmatični kortizol je određivan radioimunokemijskom (RIA) metodom. Dobiveni podatci međusobno su uspoređeni jednosmjernom analizom varijance. Prosječna razina plazmatičnog kortizola nazimica u drugoj i trećoj skupini bila je veća nego u kontrolnoj skupini. Pri tome je značajne statistička razlika (P < 0.05) ustanovljena prvog dana boravka u prasilištu između kontrolne i treće skupine. Prema dobivenim rezultatima protustresni učinak Baypamuna očitovao se 1. i 3. dana boravka u prasilištu