Istraživanje patoloških promjena kod celulitisa uzrokovanog bakterijom Erysipelothrix rhusiopathiae u pokusno zaraženih tovnih pilića.

Avian cellulitis is a serious problem for the commercial broiler industry. Although various agents were isolated from cellulitis lesions, the ability of Erysipelothrix rhusiopathiae in reproduction of cellulitis is not known. Therefore, the objective of this study was to evaluate the ability of these bacteria for induction of avian cellulitis. The study involved two experimental groups, each consisting of twenty randomly selected 15-day-old, mixed sex, healthy, commercially grown broiler chickens of the same strain. A single scratch was induced with a 1.5-inch 18-gauge needle, creating a lesion 2 cm in length on the right dorsolateral surface of the body parallel to the vertebrae. In Group 1, 1ml of 10² bacterial suspension - isolated from a turkey cock with erysipelas - was inoculated into the scratches. Birds in Group 2 received 1ml of the sterile phosphate-buffered saline as negative controls. At 48-hr post inoculation the birds were killed and pathologic and bacteriologic examinations were carried out. Birds in Group1 showed weakness, depression and mild diarrhoea. In this group, 65% of birds showed swelling of the skin with necrosis, infiltration of heterophils and fibrinous exudates, which was characteristic of cellulitis. The remaining 35% of birds were dead within 24-hrs post inoculation, with signs of mild cellulitis. Erysipelothrix rhusiopathiae was recovered from skin and some other internal organs from birds in Group 1. Birds in Group 2 were normal. Results of the present study revealed that E. rhusiopathiae can be considered as a causative agent of avian cellulitis, with public health hazards.Celulitis peradi ozbiljan je problem u proizvodnji tovnih pilića. Iako su različiti agensi izdvojeni iz celulitičnih lezija, sposobnost Erysipelothrix rhusiopathiae u izazivanju celulitisa nije poznata. Stoga je cilj ovog istraživanja bio istražiti ulogu ove bakterije u nastanku celulitisa peradi. Korištene su dvije pokusne skupine, od kojih je svaka bila sastavljena od nasumce odabranih dvadeset komercijalno uzgajanih, zdravih, petnaestodnevnih tovnih pilića, mješovita spolnog sastava i istog soja. Na desnoj dorzolateralnoj strani tijela peralelno s kralježnicom, iglom je načinjena ogrebotina duga 2 cm. Pilićima jedne skupine u tu je ogrebotinu inokuliran 1 ml suspenzije s 102 bakterijskih stanica uzročnika izdvojenog iz purana s vrbancem. Pilići druge skupine primili su 1 ml sterilne puferirane otopine fosfatne soli kao negativnu kontrolu. Pilići su bili žrtvovani 48 sati nakon inokulacije, a potom su načinjena patološka i bakteriološka istraživanja. U 65% pilića iz prve skupine uočeno je otečenje kože s nekrozama, infiltracija heterofila i fibrinozni eksudat, što je osebujno za celulitis. Preostalih 35% pilića je uginulo unutar 24 sata nakon inokulacije sa znakovima blagog celulitisa. Erysipelothrix rhusiopathiae bio je ustanovljen u koži i nekim unutarnjim organima pilića iz prve skupine. Pilići iz druge skupine bili su zdravi. Rezultati ovog istraživanja pokazuju da se E. rhusiopathiae može smatrati uzročnikom celulitisa peradi i predstavlja opasnost za javno zdravstvo