Growth of ABT-5 bacteria culture in whey supplemented with whey protein concentrate

Cilj ovog istraživanja bio je skratiti trajanje fermentacije sirutke te ispitati utjecaj dodatka koncentrata proteina sirutke (KPS) na rast i aktivnost te preživljavanje bakterija združene ABT-5 kulture (Lactobacillus acidophilus, Bifidobacterium spp. i Streptococcus thermophilus) tijekom 28 dana hladnog čuvanja (5 °C) fermentirane sirutke. Praćena je fermentacija sirutke pri 37 °C bez i s dodatkom 1,5 i 3 % KPS. Fermentacija sirutke sa združenom ABT-5 bakterijskom kulturom trajala je oko 6,5 sati, a KPS nije utjecao na dinamiku fermentacije, bez obzira na dodanu količinu. Broj živih stanica Lactobacillus acidophilus bio je veći u fermentiranoj sirutki s KPS (oko 7,13 log CFU/mL) u odnosu na fermentiranu sirutku bez KPS (oko 6,63 log CFU/mL). Dodatak KPS nije utjecao na rast bakterija Bifidobacterium spp. i Streptococcus thermophilus kao ni na preživljavanje bakterija ABT-5 kulture u fermentiranoj sirutki tijekom 28 dana hladnog čuvanja (5°C).The objective of this research was to decrease the duration of whey fermentation and to analyze the impact of whey protein concentrates (WPC) addition on growth and activity as well as on survival of mixed ABT-5 culture bacteria (Lactobacillus acidophilus, Bifidobacterium spp. and Streptococcus thermophilus) during 28 days of fermented whey cold storage (5 °C). Whey fermentation was observed at 37 °C with and without 1.5 and 3 % WPC addition. Whey fermentation with mixed ABT-5 bacterial culture took about 6.5 hours, and WPC did not affect the fermentation dynamics, regardless of the added amount. The viable cell count of Lactobacillus acidophilus was higher in fermented whey with WPC (about 7.13 log CFU/mL) compared to whey without WPC (about 6.63 log CFU/mL). WPC addition did not affect the growth of bacteria Bifidobacterium spp. and Streptococcus thermophilus nor the survival of ABT-5 culture bacteria in fermented whey during 28 days of cold storage (5 °C)