Add to ORKGZebrafish is a powerful vertebrate model system for studying development, modeling disease, and performing drug screening. Recently a variety of genetic tools have been introduced, including multiple strategies for inducing mutations and generating transgenic lines. However, large-scale screening is limited by traditional genotyping methods, which are time-consuming and labor-intensive. Here we describe a technique to analyze zebrafish genotypes by PCR combined with high-resolution melting analysis (HRMA). This approach is rapid, sensitive, and inexpensive, with lower risk of contamination artifacts. Genotyping by PCR with HRMA can be used for embryos or adult fish, including in high-throughput screening protocols.DP2 MH100008/DP/NCCDPHP CD...
Background: The CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign ...
The zebrafish (Danio rerio) is an ideal organism to study vertebrate development and model human dis...
Although zebrafish was introduced as a laboratory model organism several decades ago and now serves ...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
Color poster with text, images, charts, and graphs.Seventy percent of human genes have at least one ...
Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to ...
With the goal to generate and characterize the phenotypes of null alleles in all genes within an org...
<div><p>With the goal to generate and characterize the phenotypes of null alleles in all genes withi...
thesisZebrafish (Danio rerio) have similar genetic structure to humans and share 70% of human genes....
Zebrafish is a powerful animal model used to study vertebrate embryogenesis, organ development and d...
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A...
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A...
Background: The CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign ...
The zebrafish (Danio rerio) is an ideal organism to study vertebrate development and model human dis...
Although zebrafish was introduced as a laboratory model organism several decades ago and now serves ...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas...
Color poster with text, images, charts, and graphs.Seventy percent of human genes have at least one ...
Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to ...
With the goal to generate and characterize the phenotypes of null alleles in all genes within an org...
<div><p>With the goal to generate and characterize the phenotypes of null alleles in all genes withi...
thesisZebrafish (Danio rerio) have similar genetic structure to humans and share 70% of human genes....
Zebrafish is a powerful animal model used to study vertebrate embryogenesis, organ development and d...
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A...
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A...
Background: The CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign ...
The zebrafish (Danio rerio) is an ideal organism to study vertebrate development and model human dis...
Although zebrafish was introduced as a laboratory model organism several decades ago and now serves ...