AIDS

BackgroundCytomegalovirus (CMV) infection can be acquired in utero or postnatally through horizontal transmission and breastfeeding. The effect of postnatal CMV infection on postnatal HIV transmission is unknown.MethodsThe Breastfeeding, Antiretrovirals and Nutrition study, conducted in Malawi, randomized 2369 mothers and their infants to three antiretroviral prophylaxis arms \u2013mother (triple regimen), infant (nevirapine), or neither \u2013 for 28 weeks of breastfeeding, followed by weaning. Stored plasma and peripheral blood mononuclear cell specimens were available for 492 infants at 24 weeks and were tested with CMV PCR. Available samples from infants who were CMV PCR-positive at 24 weeks were also tested at birth (N = 242), and from infants PCR-negative at 24 weeks were tested at 48 weeks (N = 96). Cox proportional-hazards models were used to determine if CMV infection was associated with infant morbidity, mortality, or postnatal HIV acquisition.ResultsAt 24 weeks of age, CMV DNA was detected in 345/492 infants (70.1%); the estimated congenital CMV infection rate was 2.3%, and the estimated rate of CMV infection at 48 weeks was 78.5%. CMV infection at 24 weeks was associated with subsequent HIV acquisition through breastfeeding or infant death between 24 and 48 weeks of age (hazard ratio 4.27, P = 0.05).ConclusionMost breastfed infants of HIV-infected mothers in this resource-limited setting are infected with CMV by 24 weeks of age. Early CMV infection may be a risk factor for subsequent infant HIV infection through breastfeeding, pointing to the need for comprehensive approaches in order to achieve elimination of breastfeeding transmission of HIV.20152016-04-24T00:00:00Z2-D43 TW01039-06/TW/FIC NIH HHS/United StatesD43 TW001039/TW/FIC NIH HHS/United StatesP30 AI050410/AI/NIAID NIH HHS/United StatesR24 TW00798/TW/FIC NIH HHS/United StatesR24 TW007988/TW/FIC NIH HHS/United StatesU48-CCU409660-09/PHS HHS/United StatesU48-DP000059-01/DP/NCCDPHP CDC HHS/United StatesU48-DP001944-01/DP/NCCDPHP CDC HHS/United States25985405PMC4493887841