Add to ORKGTools for genetically-determined visualization of synaptic circuits and interactions are necessary to build connectomics of the vertebrate brain and to screen synaptic properties in neurological disease models. Here we develop a transgenic FingR (fibronectin intrabodies generated by mRNA display) technology for monitoring synapses in live zebrafish. We demonstrate FingR labeling of defined excitatory and inhibitory synapses, and show FingR applicability for dissecting synapse dynamics in normal and disease states. Using our system we show that chronic hypoxia, associated with neurological defects in preterm birth, affects dopaminergic neuron synapse number depending on the developmental timing of hypoxia.DP2 MH100008/DP/NCCDPHP CDC HHS/Unit...
Over recent years, several groundbreaking techniques have been developed that allow for the anatomic...
The brain is our most complex organ, with an estimated 1011 neurons. With the spinal cord, it forms ...
Understanding brain function requires the identification of neuronal connections that mediate cognit...
SummaryThe ability to visualize endogenous proteins in living neurons provides a powerful means to i...
Chemical exposures have been implicated as environmental risk factors that interact with genetic sus...
Proper function of the nervous system requires the precise wiring of neuronal circuitry, which is es...
Proper function of the nervous system requires the precise wiring of neuronal circuitry, which is es...
Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membra...
Abstract The retinotectal synapse in larval zebrafish, combined with live time-lapse imaging, provid...
A central goal of modern neuroscience is to obtain a mechanistic understanding of higher brain funct...
A central goal of Neuroscience is to understand how the brain processes sensory stimuli and generate...
The pathophysiology of most neurodegenerative diseases includes aberrant accumulation of protein agg...
A greater understanding of brain function requires tools to unravel the neuronal connections that un...
System xc– is an amino acid transporter that enables the transfer of intracellular glutamate in exch...
Brain development requires a coordinated genetic code to regulate initial cell identity determinatio...
Over recent years, several groundbreaking techniques have been developed that allow for the anatomic...
The brain is our most complex organ, with an estimated 1011 neurons. With the spinal cord, it forms ...
Understanding brain function requires the identification of neuronal connections that mediate cognit...
SummaryThe ability to visualize endogenous proteins in living neurons provides a powerful means to i...
Chemical exposures have been implicated as environmental risk factors that interact with genetic sus...
Proper function of the nervous system requires the precise wiring of neuronal circuitry, which is es...
Proper function of the nervous system requires the precise wiring of neuronal circuitry, which is es...
Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membra...
Abstract The retinotectal synapse in larval zebrafish, combined with live time-lapse imaging, provid...
A central goal of modern neuroscience is to obtain a mechanistic understanding of higher brain funct...
A central goal of Neuroscience is to understand how the brain processes sensory stimuli and generate...
The pathophysiology of most neurodegenerative diseases includes aberrant accumulation of protein agg...
A greater understanding of brain function requires tools to unravel the neuronal connections that un...
System xc– is an amino acid transporter that enables the transfer of intracellular glutamate in exch...
Brain development requires a coordinated genetic code to regulate initial cell identity determinatio...
Over recent years, several groundbreaking techniques have been developed that allow for the anatomic...
The brain is our most complex organ, with an estimated 1011 neurons. With the spinal cord, it forms ...
Understanding brain function requires the identification of neuronal connections that mediate cognit...