Add to ORKGSubstrates are targeted for proteasomal degradation through the attachment of ubiquitin chains that need to be removed by proteasomal deubiquitinases before substrate processing. In budding yeast, the deubiquitinase Ubp6 trims ubiquitin chains and affects substrate processing by the proteasome, but the underlying mechanisms and the location of Ubp6 within the holoenzyme have been elusive. Here we show that Ubp6 activity strongly responds to interactions with the base ATPase and the conformational state of the proteasome. Electron microscopy analyses reveal that ubiquitin-bound Ubp6 contacts the N ring and AAA+ ring of the ATPase hexamer and is in proximity to the deubiquitinase Rpn11. Ubiquitin-bound Ubp6 inhibits substrate deubiquitination...
The ubiquitin-proteasome system is largely responsible for the turnover of proteins in theeukaryotic...
Protein homeostasis is largely dependent on proteolysis by the ubiquitin-proteasome system. Diverse ...
SummaryThe ubiquitin ligase Hul5 was recently identified as a component of the proteasome, a multisu...
Cell survival fundamentally depends on protein degradation, which in eukaryotes is carried out to a ...
SummaryUbiquitin chains serve as a recognition motif for the proteasome, a multisubunit protease, wh...
The interplay of the proteasome and deubiquitinase Ubp6 is crucial for the degradation of ubiquityla...
The 26S proteasome is responsible for the selective, ATP-dependent degradation of polyubiquitinated ...
In eukaryotic cells, the 26S proteasome is responsible for the regulated degradation of intracellula...
The ubiquitin (Ub) system recognizes degradation signals of the target proteins through the E3 compo...
The ubiquitin–proteasome system is responsible for the bulk of protein degradation in eukaryotic cel...
The 26S proteasome is responsible for selective protein degradation in eukaryotic cells. Polyubiquit...
The proteasome is the major ATP-dependent protease in eukaryotic cells, but limited structural infor...
The 26S proteasome mediates degradation of ubiquitin-conjugated proteins. Although ubiquitin is recy...
The ATP-dependent degradation of polyubiquitylated proteins by the 26S proteasome is essential for t...
SummaryAll seven lysine residues in ubiquitin contribute to the synthesis of polyubiquitin chains on...
The ubiquitin-proteasome system is largely responsible for the turnover of proteins in theeukaryotic...
Protein homeostasis is largely dependent on proteolysis by the ubiquitin-proteasome system. Diverse ...
SummaryThe ubiquitin ligase Hul5 was recently identified as a component of the proteasome, a multisu...
Cell survival fundamentally depends on protein degradation, which in eukaryotes is carried out to a ...
SummaryUbiquitin chains serve as a recognition motif for the proteasome, a multisubunit protease, wh...
The interplay of the proteasome and deubiquitinase Ubp6 is crucial for the degradation of ubiquityla...
The 26S proteasome is responsible for the selective, ATP-dependent degradation of polyubiquitinated ...
In eukaryotic cells, the 26S proteasome is responsible for the regulated degradation of intracellula...
The ubiquitin (Ub) system recognizes degradation signals of the target proteins through the E3 compo...
The ubiquitin–proteasome system is responsible for the bulk of protein degradation in eukaryotic cel...
The 26S proteasome is responsible for selective protein degradation in eukaryotic cells. Polyubiquit...
The proteasome is the major ATP-dependent protease in eukaryotic cells, but limited structural infor...
The 26S proteasome mediates degradation of ubiquitin-conjugated proteins. Although ubiquitin is recy...
The ATP-dependent degradation of polyubiquitylated proteins by the 26S proteasome is essential for t...
SummaryAll seven lysine residues in ubiquitin contribute to the synthesis of polyubiquitin chains on...
The ubiquitin-proteasome system is largely responsible for the turnover of proteins in theeukaryotic...
Protein homeostasis is largely dependent on proteolysis by the ubiquitin-proteasome system. Diverse ...
SummaryThe ubiquitin ligase Hul5 was recently identified as a component of the proteasome, a multisu...