Environ Health Perspect

BackgroundEngineered nanomaterials (ENMs) incorporated into toner formulations of printing equipment become airborne during consumer use. Although information on the complex physicochemical and toxicological properties of both toner powders and printer-emitted particles (PEPs) continues to grow, most toxicological studies have not used the actual PEPs but rather have primarily used raw toner powders, which are not representative of current exposures experienced at the consumer level during printing.ObjectivesWe assessed the biological responses of a panel of human cell lines to PEPs.MethodsThree physiologically relevant cell lines\ue2\u20ac\u201dsmall airway epithelial cells (SAECs), macrophages (THP-1 cells), and lymphoblasts (TK6 cells)\ue2\u20ac\u201dwere exposed to PEPs at a wide range of doses (0.5\ue2\u20ac\u201c100 \uce\ubcg/mL) corresponding to human inhalation exposure durations at the consumer level of 8 hr or more. Following treatment, toxicological parameters reflecting distinct mechanisms were evaluated.ResultsPEPs caused significant membrane integrity damage, an increase in reactive oxygen species (ROS) production, and an increase in pro-inflammatory cytokine release in different cell lines at doses equivalent to exposure durations from 7.8 to 1,500 hr. Furthermore, there were differences in methylation patterns that, although not statistically significant, demonstrate the potential effects of PEPs on the overall epigenome following exposure.ConclusionsThe in vitro findings obtained in this study suggest that laser printer\ue2\u20ac\u201cemitted engineered nanoparticles may be deleterious to lung cells and provide preliminary evidence of epigenetic modifications that might translate to pulmonary disorders.CitationPirela SV, Miousse IR, Lu X, Castranova V, Thomas T, Qian Y, Bello D, Kobzik L, Koturbash I, Demokritou P. 2016. Effects of laser printer\ue2\u20ac\u201cemitted engineered nanoparticles on cytotoxicity, chemokine expression, reactive oxygen species, DNA methylation, and DNA damage: a comprehensive in vitro analysis in human small airway epithelial cells, macrophages, and lymphoblasts. Environ Health Perspect 124:210\ue2\u20ac\u201c219;\ue2\u20ac\u201ahttp://dx.doi.org/10.1289/ehp.140958226080392PMC474908