BMC Infect Dis

BackgroundThe use of molecular diagnostic techniques for the evaluation of the impact of pneumococcal conjugate vaccines (PCVs) has not been documented. We aimed to evaluate the impact of PCVs on invasive pneumococcal disease (IPD) using polymerase chain reaction (PCR)-based techniques and compare with results obtained from culture-based methods.MethodsWe implemented two independent surveillance programs for IPD among individuals hospitalized at one large surveillance site in Soweto, South Africa during 2009\u20132012: (i) PCR-based (targeting the lytA gene) syndromic pneumonia surveillance; and (ii) culture-based laboratory surveillance. Positive samples were serotyped. The molecular serotyping assay included targets for 42 serotypes including all serotypes/serogroups included in the 7-valent (PCV-7) and 13-valent (PCV-13) PCV. The Quellung reaction was used for serotyping of culture-positive cases. We calculated the change in rates of IPD (lytA- or culture-positive) among HIV-uninfected children aged <2\ua0years from the year of PCV-7 introduction (2009) to the post-vaccine years (2011 or 2012).ResultsDuring the study period there were 607 lytA-positive and 1,197 culture-positive cases that were serotyped. Samples with lytA cycle threshold (Ct)-values 6535 (30.2\ua0%; 123/407) were significantly less likely to have a serotype/serogroup detected for serotypes included in the molecular serotyping assay than those with Ct-values <35 (78.0\ua0%; 156/200) (p\u2009<\u20090.001). From 2009 to 2012 rates of PCV-7 serotypes/serogroups decreased 1263.8\ua0% (95\ua0% CI: 1279.3\ua0% to 1239.1\ua0%) among lytA-positive cases and 1291.7\ua0% (95\ua0% CI: 1298.8\ua0% to 1273.6\ua0%) among culture-positive cases. Rates of lytA-positive non-vaccine serotypes/serogroups also significantly decreased ( 1271.7\ua0%; 95\ua0% CI: 1281.1\ua0% to 1258.5\ua0%) over the same period. Such decline was not observed among the culture-positive non-vaccine serotypes (1.2\ua0%; 95\ua0% CI: 1296.7\ua0% to 58.4\ua0%).ConclusionsSignificant downward trends in IPD PCV-7 serotype-associated rates were observed among patients tested by PCR or culture methods; however trends of non-vaccine serotypes/serogroups differed between the two groups. Misclassifications of serotypes/serogroups, affecting the use of non-vaccine serotypes as a control group, may have occurred due to the low performance of the serotyping assay among lytA-positive cases with high Ct-values. Until PCR methods improve further, culture methods should continue to be used to monitor the effects of PCV vaccination programs on IPD incidence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-015-1198-z) contains supplementary material, which is available to authorized users.20152015-10-26T00:00:00Z26496761PMC4620746660