Ispitivanje različitih organskih supstrata i razvoj prikladne jeftine fermentacijske podloge u proizvodnji α-amilaze s pomoću Bacillus subtilis

The production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), water melon husk (WMH), lentil bran (LB), wheat bran (WB), melon husk (MH) and maize oil cake (MOC), different incubation time (24–144 h), particle size (500–2500 μm), initial moisture content of the substrate (40–70 %, by mass per volume), inoculum size (10–60 %, by mass per volume) and inoculum concentration (10–60 %, by volume per mass). The maximum production of amylase (4857 U/mg) was achieved when 1 % starch (by mass, particle size of 1500 μm) was added to banana husk as the solid substrate, fermented for 72 h at 37 °C, at an inoculum level of 30 % (by volume per mass), and initial moisture content of 60 % (by volume per mass).U radu je ispitana proizvodnja ekstracelularne amilaze fermentacijom na čvrstoj podlozi s pomoću Bacillus subtilis. Optimirani su različiti parametri za maksimalnu proizvodnju amilaze. Ispitani su razni čvrsti supstrati, kao što su kora banane, lubenice i dinje; mekinje leće i pšenice te kukuruzna uljna pogača; zatim vrijeme inkubacije (24-144 h), veličina čestica (500-2500 μm), početna vlažnost supstrata (40-70 %, V/m) i veličina inokuluma (10-60 %, V/m). Maksimalna proizvodnja amilaze (4857 U/mg) postignuta je dodatkom škroba (masenog udjela 1 %, veličine čestica 1500 μm) podlozi od kore banane, fermentirane tijekom 72 h na 37 °C, pri veličini inokuluma od 30 % te početnoj vlažnosti od 60 %